MICROSOMAL ALDEHYDE DEHYDROGENASE OR ITS CROSS-REACTING PROTEIN EXISTS IN OUTER MITOCHONDRIAL-MEMBRANES AND PEROXISOMAL MEMBRANES IN RAT-LIVER

被引：12

作者：

MIYAUCHI, K

YAMAMOTO, A

MASAKI, R

FUJIKI, Y

TASHIRO, Y

机构：

[1] KANSAI MED UNIV, DEPT PHYSIOL, OSAKA 570, JAPAN

[2] KANSAI MED UNIV, LIVER RES CTR CELL BIOL, OSAKA 570, JAPAN

[3] MEIJI INST HLTH SCI, MOLEC CELL BIOL LAB, ODAWARA 250, JAPAN

来源：

CELL STRUCTURE AND FUNCTION | 1993年 / 18卷 / 06期

关键词：

MICROSOMAL ALDEHYDE DEHYDROGENASE (MSALDH); OUTER MITOCHONDRIAL MEMBRANES; PEROXISOMES; IMMUNOGOLD ELECTRON MICROSCOPY;

D O I：

10.1247/csf.18.427

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the subcellular distribution of microsomal aldehyde dehydrogenase (msALDH) in rat liver and revealed by the immunoblotting method that msALDH or a cross-reacting 54-kDa protein(s) exists in the outer mitochondrial membranes and peroxisomes. Anti-msALDH antibody markedly inhibited the decanal aldehyde dehydrogenase activity of the outer mitochondrial membranes as well as that of the microsomes. Immunogold electron microscopic observations showed that gold particles are localized over the ER, outer mitochondrial membranes and peroxisomal membranes. These results suggest that msALDH or its crossreacting related protein is distributed not only in the ER membranes but also in the mitochondrial outer membranes and peroxisomal membranes.

引用

页码：427 / 436

页数：10

共 29 条

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