SEROTONIN RELEASE AND CELL-PROLIFERATION ARE UNDER THE CONTROL OF ALPHA-BUNGAROTOXIN-SENSITIVE NICOTINIC RECEPTORS IN SMALL-CELL LUNG-CARCINOMA CELL-LINES

被引：81

作者：

CODIGNOLA, A ^{[1
]}

TARRONI, P ^{[1
]}

CATTANEO, MG ^{[1
]}

VICENTINI, LM ^{[1
]}

CLEMENTI, F ^{[1
]}

SHER, E ^{[1
]}

机构：

[1] UNIV MILAN,DEPT MED PHARMACOL,I-20129 MILAN,ITALY

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

HUMAN SMALL CELL LUNG CARCINOMA; NICOTINIC ACETYLCHOLINE RECEPTOR; SEROTONIN; SECRETION; PROLIFERATION;

D O I：

10.1016/0014-5793(94)80518-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuronal type nicotinic acetylcholine receptors (nAchRs) have recently been identified in small-cell lung carcinoma. We here show that both nicotine and cytisine stimulate [H-3]serotonin release in a dose-dependent manner; this effect is antagonized by alpha-bungarotoxin (alpha Bgtx) and alpha-conotoxin MI (alpha Ctx). Nicotine and cytisine stimulate in vitro SCLC proliferation and this effect is completely antagonized by both alpha Bgtx and alpha Ctx. By PCR analysis, we demonstrate the presence in SCLC of both the alpha(7) and the beta(2) nAchR subunits mRNA. These data show that nAchRs play an important role in the biology of SCLC, and that alpha Bgtx-sensitive receptors of the alpha(7) subtype are crucially involved in both the secretagogue and mitogenic effects of nicotinic agonists.

引用

页码：286 / 290

页数：5

共 20 条

[1]

CATTANEO MG, 1993, CANCER RES, V53, P5566

[2]

CHIAPPINELLI VA, 1993, NATURAL SYNTHETIC NE, P65

[3] NEURONAL-TYPE ALPHA-BUNGAROTOXIN RECEPTORS AND THE ALPHA-5-NICOTINIC RECEPTOR SUBUNIT GENE ARE EXPRESSED IN NEURONAL AND NONNEURONAL HUMAN CELL-LINES [J].