EXPRESSION OF THE ALCALIGENES-EUTROPHUS PHBA GENE IN ESCHERICHIA-COLI USING A POSITIVE SELECTION VECTOR BASED ON PHAGE-LAMBDA LYSIS GENES

被引：6

作者：

KALOUSEK, S ^{[1
]}

SCHROT, G ^{[1
]}

LUBITZ, W ^{[1
]}

BLASI, U ^{[1
]}

机构：

[1] UNIV VIENNA,BIOCTR,INST MICROBIOL & GENET,DR BOHR GASSE 9,A-1030 VIENNA,AUSTRIA

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 33卷 / 01期

关键词：

POSITIVE SELECTION; LYSIS GENE; A-EUTROPHUS; BETA-KETOTHIOLASE;

D O I：

10.1016/0168-1656(94)90094-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new Positive selection vector, pGS23, based on the Lambda lysis cassette has been designed for efficient expression of homologous and heterologous genes in Escherichia coli. The plasmid permits controlled expression of a gene of interest under transcriptional control of the lac promoter with translation initiation of coding sequences directed by the phage T7 gene 10 ribosome binding site, The application of the vector system was tested for high level expression of the heterologous phbA gene of Alcaligenes eutrophus in E. coli.

引用

页码：15 / 19

页数：5

共 19 条

[1] THE LETHAL LAMBDA-S GENE ENCODES ITS OWN INHIBITOR [J].

BLASI, U ;

CHANG, CY ;

ZAGOTTA, MT ;

NAM, K ;

YOUNG, R .

EMBO JOURNAL, 1990, 9 (04) :981-989

[2] DUAL TRANSLATIONAL INITIATION SITES CONTROL FUNCTION OF THE LAMBDA-S GENE [J].

BLASI, U ;

NAM, K ;

HARTZ, D ;

GOLD, L ;

YOUNG, R .

EMBO JOURNAL, 1989, 8 (11) :3501-3510

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

DAVIS JT, 1987, J BIOL CHEM, V262, P82

[5] LETHAL ACTION OF BACTERIOPHAGE-GAMMA S-GENE [J].

GARRETT, JM ;

YOUNG, R .

JOURNAL OF VIROLOGY, 1982, 44 (03) :886-892

[6] USE OF THE LYSIS GENE OF BACTERIOPHAGE PHI-X174 FOR THE CONSTRUCTION OF A POSITIVE SELECTION VECTOR [J].

HENRICH, B ;

PLAPP, R .

GENE, 1986, 42 (03) :345-349

[7]

KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367

[8] A SYSTEM FOR SHOTGUN DNA SEQUENCING [J].

MESSING, J ;

CREA, R ;

SEEBURG, PH .

NUCLEIC ACIDS RESEARCH, 1981, 9 (02) :309-321

[9]

Miller J., 1972, EXPT MOL GENETICS

[10] THE T7 PHAGE GENE 10 LEADER RNA, A RIBOSOME-BINDING SITE THAT DRAMATICALLY ENHANCES THE EXPRESSION OF FOREIGN GENES IN ESCHERICHIA-COLI [J].

OLINS, PO ;

DEVINE, CS ;

RANGWALA, SH ;

KAVKA, KS .

GENE, 1988, 73 (01) :227-235

← 1 2 →