ARECOLINE-STIMULATED BRAIN INCORPORATION OF INTRAVENOUSLY ADMINISTERED FATTY-ACIDS IN UNANESTHETIZED RATS

Brain incorporation of [1-C-14]arachidonate ([C-14]AA; 170 mu-Ci/kg), [1-C-14]docosahexaenoate ([C-14]DA; 100 mu-Ci/kg), or [9,10-H-3]palmitate ([H-3]PA; 6.4 mCi/kg) infused intravenously for 5 min was examined in the awake rat following systemic administration of the cholinomimetic arecoline (15 mg/kg i.p.). The rat was killed 15 min after infusion, and the brain was removed, frozen, and prepared for biochemical analysis and autoradiography. Brain radioactivity, normalized for plasma exposure, was increased by 41 and 45% in arecoline-treated rats given [C-14]AA and [C-14]DA, respectively. Pretreatment with atropine prevented the increase in fatty acid incorporation. Arecoline treatment had no effect on brain incorporation of [H-3]PA. Quantitative autoradiography indicated regionally selective increases in brain [C-14]AA and [C-14]DA incorporation in response to arecoline. The results suggest that intravenously administered radiolabeled fatty acids can be used to study neurotransmitter-stimulated brain lipid metabolism in vivo.