DOUBLE INSITU HYBRIDIZATION FOR DETECTION OF HERPES-SIMPLEX VIRUS AND CYTOMEGALOVIRUS DNA USING NONRADIOACTIVE PROBES

被引：15

作者：

GENTILOMI, G ^{[1
]}

MUSIANI, M ^{[1
]}

ZERBINI, M ^{[1
]}

GIBELLINI, D ^{[1
]}

GALLINELLA, G ^{[1
]}

VENTUROLI, S ^{[1
]}

机构：

[1] UNIV BOLOGNA,INST MICROBIOL,VIA MASSARENTI 9,I-40138 BOLOGNA,ITALY

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1992年 / 40卷 / 03期

关键词：

DOUBLE HYBRIDIZATION; HSV/CMV DNA; BIOTINYLATED AND DIGOXIGENIN-LABELED PROBES;

D O I：

10.1177/40.3.1313062

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a double in situ hybridization assay for the simultaneous detection of Herpes simplex virus (HSV) and cytomegalovirus (CMV) DNA in, infected cell cultures using nonradioactive-labeled probes. This work used a biotinylated HSV DNA probe, which can be revealed by an avidin-biotin-peroxidase complex and a digoxigenin-labeled CMV DNA probe, visualized by anti-digoxigenin F(ab) fragments conjugated with alkaline phosphatase. Light microscopy visualization was achieved by the contrasting colors of appropriate peroxidase and alkaline phosphatase reaction products (red and dark blue, respectively). The time required to perform the double hybridization assay was about 3 hr. This double hybridization assay proved to be sensitive, specific, and provided good resolving power.

引用

页码：421 / 425

页数：5

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