LOCALIZATION AND DEVELOPMENTAL EXPRESSION OF A NOVEL PROTEIN-KINASE-C DELTA-GENE

The expression and localization of a novel protein kinase C delta (nPKCdelta) mRNA were investigated using Northern blotting and in situ hybridization in the developmental process of mouse brain. In adult mice, nPKCdelta was abundantly expressed in the thalamus, moderately in the pons and the cerebellum, but faintly in the cerebral cortex and the spinal cord. By in situ hybridization, the signals were observed specifically at the sensory and motor relay nuclei of the thalamus, the dorsal cochlear nuclei of the pons, and the molecular layer of the cerebellum. When developmental changes in the expresssion of nPKCdelta gene were analyzed by in situ hybridization, it was not detectable in embryonic and neonatal brains, very weakly expressed in the thalamus in the first week, and highly expressed at two weeks of age. These results suggest that the gene expression of nPKCdelta is strictly controlled by both the cell type and the developmental process.