ANALYSIS OF INDIVIDUAL PHOSPHOLIPIDS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

A method of analysis for determination of phospholipids by micellar electrokinetic capillary chromatography (MECC) has been developed. Sodium cholate (NaCh) was found suitable as the micellar phase, and 1-propanol was important in obtaining an efficient separation of individual phospholipids and as organic solvent required for acceptable solubility of the phospholipids. With equal volatility of water and organic modifier, only minor changes in effects from the modifier occur during analysis. The influence of changes in high performance capillary electrophoresis-MECC separation conditions were evaluated in terms of migration time, relative migration time (RMT), relative normalized area (RNA), resolution and theoretical plates per meter. The method has several advantages compared to high-performance liquid chromatography: The total time of analysis is less than 25 min, and only small amounts of reagents and sample are required. Relative standard deviations were 0.26-0.48% for RMT 1.7-2.9% for RNA, and in the linearity test correlation coefficients of 0.999 were obtained. Results from analyses of the phospholipid compositions of soybean and rapeseed lecithins are presented.