A SPECTROPHOTOMETRIC ASSAY FOR PHOSPHOLIPASE-D

被引：62

作者：

DARRIGO, P ^{[1
]}

PIERGIANNI, V ^{[1
]}

SCARCELLI, D ^{[1
]}

SERVI, S ^{[1
]}

机构：

[1] POLITECN MILAN, CNR, CTR STUDIO SOSTANZE ORGAN NAT, DIPARTIMENTO CHIM, I-20131 MILAN, ITALY

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 304卷 / 02期

关键词：

ENZYMATIC METHODS; PHOSPHOLIPASE D; SPECTROPHOTOMETRY;

D O I：

10.1016/0003-2670(94)00613-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The preparation of phosphatidyl-p-nitrophenol and its use as a substrate in the non-enzyme based assay for the quantitative evaluation of phospholipase D activity is described. The solution of the substrate in Tris buffer pH 8 and Triton X-100 is mixed directly with an aliquot of a fermentation broth or a plant extract and the absorbance of the solution at 405 nm is read. The method is rapid, accurate and inexpensive. Application in the pH range 4-9 is possible.

引用

页码：249 / 254

页数：6

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