ELECTROPHORETIC PATTERNS OF SEED GLOBULINS IN THE OLD-WORLD LUPINUS SPECIES

Electrophoretic analysis of seed globulins was performed to investigate relationships between the Old-World Lupinus species. The analysis covered 11 species described earlier and the recently reported taxon, Lupinus anatolicus nom. nud. With only two exceptions, particular species were represented by various accessions. In total, 110 accessions were examined. SDS-PAGE of reduced globulins was carried out in a discontinuous buffer system. The results showed a marked difference between the smooth-seeded and the rough-seeded species. In the group of smooth-seeded lupins pronounced interspecific differences were recorded. The rough-seeded species formed a rather homogenous group, well distinguishable from the smooth-seeded species. Though no distinct interspecific differences were recorded in the rough-seeded group, most of the 34 investigated L. atlanticus accessions could be distinguished due to a characteristic, prominent polypeptide band with approx. M(r) 27 kDa. The recently reported smooth-seeded taxon, L. anatolicus, showed a species-specific SDS-PAGE globulin pattern.