PREPARATIVE REVERSED-PHASE CHROMATOGRAPHY OF POLAR AND NONPOLAR METABOLITES ON COLUMNS PACKED WITH MICRONIZED XAD-2 RESIN

Coarse-grade XAD-2 resin has been micronized (medium particle size 12 μm) and used as a support for preparative high-resolution liquid chromatography. By equilibration with single-phase mixtures of water, a lower aliphatic alcohol and a hydrophobic solvent a reversed-phase partition system is formed in situ. This chromatographic technique is characterized by a large capacity, a high power of resolution and a high degree of selectivity and reproducibility, and versatile application in the isolation of polar and non-polar drug metabolites or naturally occurring compounds from complex mixtures is possible. The examples given relate particularly to the separation of water-soluble, non-extractable compounds, such as glucuronides or other conjugates, and amino acids on large-diameter columns (up to 2.5 cm). © 1979.