A CELLULAR FACTOR BINDS TO THE HERPES-SIMPLEX VIRUS TYPE-1 TRANSACTIVATOR VMW65 AND IS REQUIRED FOR VMW65-DEPENDENT PROTEIN-DNA COMPLEX ASSEMBLY WITH OCT-1

被引：106

作者：

XIAO, P ^{[1
]}

CAPONE, JP ^{[1
]}

机构：

[1] MCMASTER UNIV,DEPT BIOCHEM,1200 MAIN ST W,HAMILTON L8N 3Z5,ONTARIO,CANADA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 09期

关键词：

D O I：

10.1128/MCB.10.9.4974

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The herpes simplex virus transactivator Vmw65 assembles into a multicomponent protein-DNA complex along with the octamer binding protein Oct-1. Using affinity chromatography on columns conjugated with purified Vmw65 fusion protein expressed in Escherichia coli, we demonstrate that a cellular factor, distinct from Oct-1, binds to Vmw65 in the absence of target DNA and is necessary for Vmw65-mediated complex assembly with Oct-1.

引用

页码：4974 / 4977

页数：4

共 29 条

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