ISOLATION AND CHARACTERIZATION OF RECOMBINANT DNA PLASMIDS CARRYING DROSOPHILA TRANSFER-RNA GENES

被引：32

作者：

DUNN, R

DELANEY, AD

GILLAM, IC

HAYASHI, S

TENER, GM

GRIGLIATTI, T

MISRA, V

SPURR, MG

TAYLOR, DM

MILLER, RC

机构：

[1] UNIV BRITISH COLUMBIA,DEPT BIOCHEM,VANCOUVER V6T 1W5,BC,CANADA

[2] UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER V6T 1W5,BC,CANADA

[3] UNIV BRITISH COLUMBIA,DEPT MICROBIOL,VANCOUVER V6T 1W5,BC,CANADA

[4] UNIV BRITISH COLUMBIA,DEPT MED GENET,VANCOUVER V6T 1W5,BC,CANADA

来源：

GENE | 1979年 / 7卷 / 3-4期

关键词：

Drosophila melanogaster; hybridization to polytene/ chromosomes; restriction endonucleases HindIII; HaeIII; PstI; EcoRI; DNA cloning; pBR322; plasmids;

D O I：

10.1016/0378-1119(79)90046-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Recombinant plasmids carrying Drosophila melanogaster tRNA genes were constructed by ligation of HindIII-cleaved Drosophila DNA to HindIII cut pBR322 DNA. 90 clones were isolated that contained genes for one or more of eleven tRNAs. 43 of the plasmids were characterized by a number of methods: restriction nuclease digestion; agarose gel electrophoresis; hybridization with individual, purified, 125I-labelled Drosophila tRNA molecules and in situ hybridization to Drosophila chromosomes. The results show that several different tRNA genes have been isolated which code for single, specific isoacceptors. The DNAs from 8 plasmids each hybridize to single sites on Drosophila polytene chromosomes. In addition, the data show examples of two different plasmids hybridizing to different loci coding for the same tRNA; this means that we have isolated representatives of tRNA genes which map at widely separated points on the Drosophila genome. © 1979.

引用

页码：197 / 215

页数：19

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