SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS - TOWARDS AN INTEGRATED PROTEIN DATABASE

被引：55

作者：

AEBERSOLD, R ^{[1
]}

LEAVITT, J ^{[1
]}

机构：

[1] INST MED RES,SAN JOSE,CA 95128

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 07期

关键词：

D O I：

10.1002/elps.1150110702

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Improved technologies or the synergistic use of complementary methods enhance the efficiency of research and permit the exploration of new approaches for the investigation of complex problems. High sensitivity protein sequence analysis and polyacrylamide gel electrophoresis are such complementary methods. Here we summarize the current status of high sensitivity sequence analysis of proteins separated in polyacrylamide gels and discuss strategies by which this technology can cnhance biological research by generating new approaches for the solution of complex, multifacetted problems. Finally, we outline lmminent technological advances in the area of high sensitivity protein sequence analysis and argue that further technological developments will ultimately lead to the generation of an integrated protein database (containing structural and functional as well as physiological information in an easily accessible form) of all the proteins separated by high resolution two‐dimensional gel electrophoresis. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：517 / 527

页数：11

共 89 条

[1]

AEBERSOLD R, 1989, METHODS PROTEIN SEQU, P78

[2] COVALENT IMMOBILIZATION OF PROTEINS FOR HIGH-SENSITIVITY SEQUENCE-ANALYSIS - ELECTROBLOTTING ONTO CHEMICALLY ACTIVATED GLASS FROM SODIUM DODECYL-SULFATE POLYACRYLAMIDE GELS [J].