SOMATOSTATIN BINDING TO PITUITARY PLASMA-MEMBRANES

被引：59

作者：

LEITNER, JW ^{[1
]}

RIFKIN, RM ^{[1
]}

MAMAN, A ^{[1
]}

SUSSMAN, KE ^{[1
]}

机构：

[1] UNIV COLORADO,SCH MED,DEPT MED,DIV ENDOCRINOL,DENVER,CO 80220

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)92045-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the study of somatostatin binding to anterior pituitary plasma membranes. When 5×10-9M [125I]Tyr1-somatostatin (SA 18 Ci/mmol) was incubated with isolated pituitary plasma membranes (protein = 100 μg), 13.6% of total radioactivity was bound excluding nonspecific binding. The Scatchard plot could be resolved into two distinct components and analyzed to yield: K1diss = 3.3×10-8M and K2diss = 7.7×10-6M. This binding was shown to be specific for somatostatin. © 1979.

引用

页码：919 / 927

页数：9

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