ENZYMATIC TRANSFORMATION OF DESACETYL-LANATOSIDE-A INTO DIGITOXIN BY BETA-GLUCOSIDASE ACTION IN CYCLODEXTRIN SOLUTIONS

被引：8

作者：

PEKIC, B

LEPOJEVIC, Z

机构：

[1] Institute of Microbiological Processes and Applied Chemistry, Faculty of Technology, University of Novi Sad, Novi Sad, 21000

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 06期

关键词：

D O I：

10.1007/BF01030990

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic transformation of desacetyl-lanatoside A (DLA) to its secondary glycoside, digitoxin, in solutions of beta- and gamma-cyclodextrins is effected using of beta-glucosidase from barley. Due to the interaction of cyclodextrins (CyDs) with desacetyl-lanatoside A, an increase in solubility of the latter of 24.5 and 230 times was observed for beta-cyclodextrin and gamma-cyclodextrin, respectively. Kinetic studies of the enzymatic transformation gave for beta-glucosidase the values K(M) = 3.3 x 10(-4) mol dm-3 and V(max) = 0.557-mu-mol mg-1 min-1 when the substrate was the deacetyl-lanatoside A complex with beta-cyclodextrin, while in the case of the complex with gamma-cyclodextrin these values were K(M) = 5.45 x 10(-4) mol dm-3 and V(max) = 0.896-mu-mol mg-1 min-1.

引用

页码：399 / 404

页数：6