CALVASCULIN, AS A FACTOR AFFECTING THE MICROFILAMENT ASSEMBLIES IN RAT FIBROBLASTS TRANSFECTED BY SRC GENE

被引：83

作者：

WATANABE, Y ^{[1
]}

USADA, N ^{[1
]}

MINAMI, H ^{[1
]}

MORITA, T ^{[1
]}

TSUGANE, S ^{[1
]}

ISHIKAWA, R ^{[1
]}

KOHAMA, K ^{[1
]}

TOMIDA, Y ^{[1
]}

HIDAKA, H ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH MED,DEPT PHARMACOL,TSURAMAI 65,SHOWA KU,NAGOYA,AICHI 466,JAPAN

来源：

FEBS LETTERS | 1993年 / 324卷 / 01期

关键词：

CALVASCULIN; EF-HAND; F-ACTIN; MICROFILAMENT; RAT FIBROBLASTS TRANSFECTED BY SRC GENE; SR-3Y1; CELL;

D O I：

10.1016/0014-5793(93)81530-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL-98, 18A2, 42A, p9Ka, or mts1, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR-3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co-sediments with F-actin and bundles actin filaments in a Ca2+-dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR-3Y1 cell is due to the cross-linking activity of calvasculin.

引用

页码：51 / 55

页数：5

共 17 条

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