FEASIBILITY STUDY OF A DRUG IMMUNOASSAY BASED ON MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION - DETERMINATION OF THEOPHYLLINE IN SERUM

被引：40

作者：

STEINMANN, L ^{[1
]}

CASLAVSKA, J ^{[1
]}

THORMANN, W ^{[1
]}

机构：

[1] UNIV BERN,DEPT CLIN PHARMACOL,CH-3010 BERN,SWITZERLAND

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 10期

关键词：

IMMUNOASSAY; THEOPHYLLINE LASER INDUCED FLUORESCENCE DETECTION; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; SERUM;

D O I：

10.1002/elps.11501601315

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper presents principle and first results of a novel competitive binding immunoassay for monitoring of theophylline in human serum. The assay is based upon short time incubation of a mixture of antiserum, containing the antibody raised against theophylline, fluorescein labelled theophylline (tracer) and serum prior to injection of a few nanoliter of this mixture onto a fused-silica capillary for subsequent separation and analysis of free tracer and the antibody-tracer-complex by micellar electrokinetic capillary chromatography with laser induced fluorescence detection. Quantitation based upon multi-level calibration using the height of the peak produced by the free tracer is shown to provide theophylline serum levels which are in agreement with those obtained by a commercial fluorescence polarization immunoassay and with those determined by micellar elektrokinetic capillary chromatography with direct serum injection and on-column UV absorption detection.

引用

页码：1912 / 1916

页数：5

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