ANGULAR RECONSTITUTION - A POSTERIORI ASSIGNMENT OF PROJECTION DIRECTIONS FOR 3-D RECONSTRUCTION

被引：445

作者：

VANHEEL, M

机构：

[1] Fritz Haber Institute of the Max-Planck Society, D-1000 Berlin Dahlem, Germany

来源：

ULTRAMICROSCOPY | 1987年 / 21卷 / 02期

关键词：

IMAGE PROCESSING - Mathematical Models;

D O I：

10.1016/0304-3991(87)90078-7

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method of 'angular reconstitution' is described, which allows the a posteriori determination of the relative angular orientations of the projections and thus enables the three-dimensional reconstruction of the object to be calculated. For asymmetric objects, a minimum of three projections is required, which should not be related by a tilt around a single rotation axis. The method can be applied to determine the three-dimensional structure of biological macromolecules based on electron micrographs of randomly oriented individual molecules. Angular reconstitution, in combination with multivariate statistical techniques to classify and average the characteristic views of a molecule forms a complete, self-contained methodology for molecular structure analysis by electron microscopy.

引用

收藏

页码：111 / 123

页数：13

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