DETERMINATION OF AMINO-ACIDS IN HUMAN-PLASMA BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：14

作者：

BACHMANN, EW ^{[1
]}

FREI, J ^{[1
]}

MUEHLEMANN, M ^{[1
]}

机构：

[1] SEARLE SA,CH-1003 LAUSANNE,SWITZERLAND

来源：

CHROMATOGRAPHIA | 1979年 / 12卷 / 06期

关键词：

2,4-Dinitrophenylderivatives; Amino acids; High-pressure liquid chromatography; Plasma;

D O I：

10.1007/BF02259151

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The derivatization reaction of human plasma was performed with 2,4-dinitrofluorobenzen at 40 °C during 3 hours. Separation occurred on reverse-phase C-18 columns, 50 min were necessary to achieve complete elution. Exactitude, precision, sensitivity of the method were better than the conventional ion-exchange separation and ninhydrin assay procedure. © 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：345 / 348

页数：4

共 8 条

[1] TECHNICAL PITFALLS LEADING TO ERRORS IN QUANTITATION OF PLASMA AMINO ACIDS [J].