PURIFICATION AND PROPERTIES OF A LIPOLYTIC ACYL-HYDROLASE FROM POTATO LEAVES

被引：43

作者：

MATSUDA, H

HIRAYAMA, O

机构：

[1] Department of Agricultural Chemistry, Shimane University, Matsue

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 573卷 / 01期

关键词：

(Potato leaf); Acyl-hydrolase; Galactolipase; Palmitoylated gauze chromatography; Phospholipase;

D O I：

10.1016/0005-2760(79)90182-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lipolytic acyl-hydrolase was purified 520-fold from an homogenate of potato leaves (Solarium tuberosum L. cv. Benimaru). The purified enzyme showed a single protein band on polyacrylamide gel electrophoresis. The enzyme had an isoelectric point of 4.6 and a molecular weight of about 110 000. It had pH optima of 5.5 and 5.0, and Km values of 0.26 and 0.54 mM for monogalactosyldiacylglycerol and phosphatidylcholine, respectively. The pH dependences were altered by the addition of Triton X-100. No separation of these two hydrolyzing activities was achieved; the ratio of the specific activity of galactolipase to that of phospholipase (about 7 1) remained constant throughout the purification procedures. Both the activities were changed in parallel with each other by the addition of reagents and by heat treatment. The enzyme clearly catalyzed the deacylation of the several classes of galacto- and phospholipids. These results suggest that a single enzyme is responsible for both activities. © 1979.

引用

页码：155 / 165

页数：11

共 29 条

[1] ROLE OF GALACTOLIPIDS IN SPINACH CHLOROPLAST LAMELLAR MEMBRANES .1. PARTIAL-PURIFICATION OF A BEAN LEAF GALACTOLIPID LIPASE AND ITS ACTION ON SUBCHLOROPLAST PARTICLES [J].

ANDERSON, MM ;

MCCARTY, RE ;

ZIMMER, EA .

PLANT PHYSIOLOGY, 1974, 53 (05) :699-704

[2]

BROCKERHOFF H, 1971, J BIOL CHEM, V246, P5828

[3] ASPECTS OF LIPID METABOLISM IN HIGHER PLANTS .3. ENZYMIC BREAKDOWN OF LIPIDS IN POTATO TUBER BY PHOSPHOLIPID-ACYL AND GALACTOLIPID-ACYL HYDROLASE ACTIVITIES AND BY LIPOXYGENASE [J].

GALLIARD, T .

PHYTOCHEMISTRY, 1970, 9 (08) :1725-&

[4]

Galliard T., 1975, Recent advances in the chemistry and biochemistry of plant lipids. Proceedings of a symposium arranged by the Phytochemical Society and the Lipid Group of the Biochemical Society, University of East Anglia, Norwich, April, 1974., P319

[5] ENZYMIC DEACYLATION OF PHOSPHOLIPIDS AND GALACTOLIPIDS IN PLANTS - PURIFICATION AND PROPERTIES OF A LIPOLYTIC ACYL-HYDROLASE FROM POTATO TUBERS [J].