IMMOBILIZATION OF PENICILLIN ACYLASE USING POROUS POLYACRYLONITRILE FIBERS

被引：16

作者：

ISHIMURA, F

SEIJO, H

机构：

[1] Research Laboratories, Toyo Jozo Co., Ltd., Tagata-gun, Shizuoka, 410-23, Mifuku, Ohito-cho

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 71卷 / 02期

关键词：

D O I：

10.1016/0922-338X(91)90242-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immobilized penicillin acylase prepared by glutaraldehyde crosslinking on hydrogenated porous polyacrylonitrile fibers showed a high specific activity. The immobilization yield was markedly influenced by the ratio of the amount of free enzyme to the weight of the support. A specific activity of 1,910 U/(g of product) and a yield of 82% were obtained. This immobilization method for penicillin acylase was superior to other methods in specific activity and immobilization yield.

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页码：140 / 143

页数：4

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