PCR DETECTION OF THE RAT-BRAIN BASIC FIBROBLAST GROWTH-FACTOR (BFGF) MESSENGER-RNA CONTAINING A UNIQUE 3' UNTRANSLATED REGION

被引：17

作者：

ELHUSSEINI, AED ^{[1
]}

PATERSON, JA ^{[1
]}

MYAL, Y ^{[1
]}

SHIU, RPC ^{[1
]}

机构：

[1] UNIV MANITOBA,FAC MED,DEPT ANAT,WINNIPEG R3E 0W3,MANITOBA,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 03期

关键词：

BFGF; RT-PCR; MESSENGER RNA STABILITY; NOVEL SEQUENCE;

D O I：

10.1016/0167-4781(92)90031-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We utilized the reverse transcription-polymerase chain reaction (RT-PCR) to detect the presence of basic fibroblast growth factor (bFGF) messenger RNA in rat brain, ovary and kidney. The nucleotide sequence of the RT-PCR product revealed a novel 3' untranslated (UT) sequence in the rat basic FGF mRNA. In this sequence, as in the 3' UT regions of many growth regulatory genes, there is a high degree of conservation of A + T rich motifs which have been shown to play a major role in mRNA stability.

引用

页码：314 / 316

页数：3

共 15 条

[1]

AKASHI M, 1991, BLOOD, V78, P2005

[2]

BAIRD A, 1986, RECENT PROG HORM RES, V42, P143

[3] DETERMINANTS OF MESSENGER-RNA STABILITY [J].

BRAWERMAN, G .

CELL, 1987, 48 (01) :5-6

[4] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[5] Basic Fibroblast Growth Factor (FGF) in the Central Nervous System: Identification of Specific Loci of Basic FGF Expression in the Rat Brain [J].

Emoto, Naoya ;

Gonzalez, Ana-Maria ;

Walicke, Patricia A. ;

Wada, Etsuko ;

Simmons, Donna M. ;

Shimasaki, Shunichi ;

Baird, Andrew .

GROWTH FACTORS, 1989, 2 (01) :21-29

[6] ANGIOGENIC FACTORS [J].

FOLKMAN, J ;

KLAGSBRUN, M .

SCIENCE, 1987, 235 (4787) :442-447

[7] FIBROBLAST GROWTH-FACTOR [J].

GOSPODAROWICZ, D ;

NEUFELD, G ;

SCHWEIGERER, L .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1986, 46 (03) :187-204

[8] SYNERGISTIC INDUCTION OF MESODERM BY FGF AND TGF-BETA AND THE IDENTIFICATION OF AN MESSENGER-RNA CODING FOR FGF IN THE EARLY XENOPUS EMBRYO [J].

KIMELMAN, D ;

KIRSCHNER, M .

CELL, 1987, 51 (05) :869-877

[9] CLONING AND EXPRESSION OF CDNA-ENCODING HUMAN BASIC FIBROBLAST GROWTH-FACTOR [J].

KUROKAWA, T ;

SASADA, R ;

IWANE, M ;

IGARASHI, K .

FEBS LETTERS, 1987, 213 (01) :189-194

[10] NUCLEOTIDE-SEQUENCE OF RAT BASIC FIBROBLAST GROWTH-FACTOR CDNA [J].

KUROKAWA, T ;

SENO, M ;

IGARASHI, K .

NUCLEIC ACIDS RESEARCH, 1988, 16 (11) :5201-5201

← 1 2 →