REGULATION OF DIFFERENTIALLY SPLICED TRANSCRIPTS OF ACYL-COA OXIDASE IN THE RAT

RNAse protection assay was used to distinguish between and to quantify alternatively spliced transcripts of acyl-CoA oxidase in liver, kidney and testis of control and methylclofenapate treated rats. The ratio of spliced transcripts (type I to II) was 1.18:1 in control liver RNA, with 130 and 110 molecules/cell, respectively, and 3.1:1 in treated liver RNA, with 2800 and 900 molecules / cell. The ratios were 1.6 and 2:1 in control and treated kidney, and 0.31:1 in testis. This is likely to be due to differential splicing, which is, therefore, regulated during peroxisome proliferation, and also in a tissue specific fashion.