CHARACTERIZATION AND IMMUNOGENICITY OF A CANDIDATE SUBUNIT VACCINE AGAINST VARICELLA-ZOSTER VIRUS

被引：17

作者：

DAVIES, J ^{[1
]}

HALLWORTH, JA ^{[1
]}

MCLEISH, P ^{[1
]}

RANDALL, S ^{[1
]}

MARTIN, BAB ^{[1
]}

BUCHAN, A ^{[1
]}

SKINNER, GRB ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH MED,DEPT INFECT,BIRMINGHAM B15 2TJ,W MIDLANDS,ENGLAND

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 1994年 / 183卷 / 02期

关键词：

D O I：

10.1007/BF00277161

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specific glycoproteins, while virus DNA was less than 20 pg/250 mu g protein - a putative vaccine dose. The vaccine was immunogenic in rabbits and stimulated antibodies to the major capsid protein as well as to glycoproteins; an immunoprecipitin was shared with a known immune human serum. The preparation contained no infectious VZV with no evidence of side effects in a rabbit or in five human vaccinees during a follow-up period of 6 - 10 years.

引用

页码：105 / 117

页数：13

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