DNA-DEPENDENT RNA POLYMERASE FROM E COLI - STUDIES ON ROLE OF SIGMA IN CHAIN INITIATION

被引：26

作者：

DUNN, JJ

BAUTZ, EKF

机构：

[1] Institute of Microbiology, Rutgers University, The State University of New Jersey, New Brunswick

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 06期

关键词：

D O I：

10.1016/0006-291X(69)90292-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyrophosphate exchange reaction catalyzed by E. coli RNA polymerase on phage T4 and calf thymus DNA templates was studied as a measure of synthesis of the initiating dinucleotide. The results indicate that the E. coli σ protein functions at a step prior to the completion of the first phosphodiester bond. Furthermore, incorporation of ATP into the 5′ terminus of RNA chains appears to be more strongly dependent on σ than incorporation of GTP. © 1969.

引用

页码：925 / &

共 7 条

[1] DNA-DEPENDENT RNA POLYMERASE FROM PHAGE T4 INFECTED E COLI - AN ENZYME MISSING A FACTOR REQUIRED FOR TRANSCRIPTION OF T4 DNA [J].