INVITRO FERTILIZATION OF INVITRO-MATURED EQUINE OOCYTES

被引：48

作者：

DELCAMPO, MR

DONOSO, MX

PARRISH, JJ

GINTHER, OJ

机构：

[1] Department of Veterinary Science, University of Wisconsin-Madison, Madison, Wisconsin

[2] Department of Meat and Animal Science, University of Wisconsin-Madison, Madison, Wisconsin

来源：

JOURNAL OF EQUINE VETERINARY SCIENCE | 1990年 / 10卷 / 01期

关键词：

D O I：

10.1016/S0737-0806(06)80078-X

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Oocytes were recovered from ovarian follicles and matured in vitro for 48 h. Sperm were capacitated by a procedure that used a Percoll wash and exposure to caffeine or caffeine plus the calcium ionophore A23187. The ability of sperm to fertilize oocytes in vitro was not different between the two treatments (p>0.05). Overall, 21/143 (15%) oocytes were fertilized in vitro. This is apparently the first report of successful in vitro fertilization of in vitro-matured equine oocytes. © 1990, All rights reserved.

引用

页码：18 / 22

页数：5

共 17 条

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