MORPHOLOGICAL CELL CHANGES DUE TO CHEMICAL TOXICITY OF A DENTAL MATERIAL - AN ELECTRON-MICROSCOPIC STUDY ON HUMAN PERIODONTAL-LIGAMENT FIBROBLASTS AND L929 CELLS

被引:48
作者
ALNAZHAN, S [1 ]
SPANGBERG, L [1 ]
机构
[1] UNIV CONNECTICUT,SCH DENT MED,DEPT RESTORAT DENT & ENDODONT,FARMINGTON,CT 06032
关键词
D O I
10.1016/S0099-2399(06)81589-X
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
New endodontic materials with polymer bases may be more difficult to evaluate in cell cultures in vitro than conventional zinc oxide-eugenol cements. In order to study the morphological changes taking place in cells exposed to such materials, L929 cells and human periodontal fibroblasts were observed using scanning electron microscopic and transmission electron microscopic techniques. The morphological changes of the cells were correlated to the quantitative results observed simultaneously in cytotoxicity studies using the radiochromium release method. Results showed there was a relationship between the chromium release and the degree of individual cell damage. The periodontal ligament fibroblasts were more resistant to this kind of chemical injury than the L929 cells. Consequently, it may be proper to use periodontally derived cells for the study of cytotoxic mechanisms of polymer endodontic filling materials. © 1990 American Association of Endodontists.
引用
收藏
页码:129 / 134
页数:6
相关论文
共 19 条
[1]  
AUTIAN J, 1970, INT DENT J, V20, P481
[2]  
AUTIAN J, 1974, INT DENT J, V24, P235
[3]  
BELKIN M, 1962, J NATL CANCER I, V28, P187
[4]   EARLY MORPHOLOGICAL CHANGES PRODUCED BY ANTI-INFLAMMATORY STEROIDS ON TISSUE CULTURE FIBROBLASTS [J].
BERLINER, DL ;
GALLEGOS, AJ ;
SCHNEEBELI, GL .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1967, 48 (01) :44-+
[5]   AGAR DIFFUSION METHOD FOR TOXICITY SCREENING OF PLASTICS ON CULTURED CELL MONOLAYERS [J].
GUESS, WL ;
ROSENBLU.SA ;
SCHMIDT, B ;
AUTIAN, J .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1965, 54 (10) :1545-&
[6]   CYTO-TOXIC EFFECTS OF DENTAL CEMENTS ON 2 CELL-CULTURE SYSTEMS [J].
HANKS, CT ;
ANDERSON, M ;
CRAIG, RG .
JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1981, 10 (02) :101-112
[7]  
KAWAHARA H, 1968, INT DENT J, V18, P443
[8]   BIOLOGICAL EVALUATION ON GLASS IONOMER CEMENT [J].
KAWAHARA, H ;
IMANISHI, Y ;
OSHIMA, H .
JOURNAL OF DENTAL RESEARCH, 1979, 58 (03) :1080-1086
[9]  
KOKUBU TATSUO, 1962, EXPTL AND MOLECULAR PATHOL, V1, P293, DOI 10.1016/0014-4800(62)90026-6
[10]  
KOSKINEN KP, 1981, SCAND J DENT RES, V89, P71