CHARACTERIZATION OF MURINE MONOCLONAL-ANTIBODIES THAT RECOGNIZE DEFINED EPITOPES OF PERTUSSIS TOXIN AND NEUTRALIZE ITS TOXIC EFFECT ON CHINESE-HAMSTER OVARY CELLS

被引：14

作者：

WALKER, MJ ^{[1
]}

WEHLAND, J ^{[1
]}

TIMMIS, KN ^{[1
]}

RAUPACH, B ^{[1
]}

SCHMIDT, MA ^{[1
]}

机构：

[1] UNIV HEIDELBERG,ZENTRUM MOLEK BIOL,W-6900 HEIDELBERG,GERMANY

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 11期

关键词：

D O I：

10.1128/IAI.59.11.4249-4251.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Three murine monoclonal antibodies (MAb), E19, E205, and E251, raised against pertussis toxin reacted in Western blots (immunoblots) with the S1, S4, and S2-S3 subunits, respectively, and neutralized the Chinese hamster ovary cell-clustering activity of pertussis toxin. MAb E251 recognized a linear synthetic peptide corresponding to amino acids 107 to 120 of the S2 subunit, suggesting a role for this region in receptor binding.

引用

页码：4249 / 4251

页数：3

共 30 条

[1] NEUTRALIZATION OF BIOLOGICAL-ACTIVITIES OF PERTUSSIS TOXIN WITH A MONOCLONAL-ANTIBODY [J].

ANWAR, H ;

ASHWORTH, LAE ;

FUNNELL, S ;

ROBINSON, A ;

IRONS, LI .

FEMS MICROBIOLOGY LETTERS, 1987, 44 (01) :141-145

[2] PERTUSSIS HOLOTOXOID FORMED INVITRO WITH A GENETICALLY DEACTIVATED S1 SUBUNIT [J].

BARTLEY, TD ;

WHITELEY, DW ;

MAR, VL ;

BURNS, DL ;

BURNETTE, WN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8353-8357

[3]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[4] ISOLATION OF PURE IGG1, IGG2A AND IGG2B IMMUNOGLOBULINS FROM MOUSE SERUM USING PROTEIN A-SEPHAROSE [J].

EY, PL ;

PROWSE, SJ ;

JENKIN, CR .

IMMUNOCHEMISTRY, 1978, 15 (07) :429-436

[5]

Francotte M., 1989, VACCINES 89, P243

[6] INTERACTION OF MONOCLONAL-ANTIBODIES WITH PERTUSSIS TOXIN AND ITS SUBUNITS [J].

FRANK, DW ;

PARKER, CD .

INFECTION AND IMMUNITY, 1984, 46 (01) :195-201

[7] THE STANDARDIZATION OF AN ASSAY FOR PERTUSSIS TOXIN AND ANTITOXIN IN MICROPLATE CULTURE OF CHINESE-HAMSTER OVARY CELLS [J].

GILLENIUS, P ;

JAATMAA, E ;

ASKELOF, P ;

GRANSTROM, M ;

TIRU, M .

JOURNAL OF BIOLOGICAL STANDARDIZATION, 1985, 13 (01) :61-&

[8] INDUCTION OF A NOVEL MORPHOLOGICAL RESPONSE IN CHINESE-HAMSTER OVARY CELLS BY PERTUSSIS TOXIN [J].

HEWLETT, EL ;

SAUER, KT ;

MYERS, GA ;

COWELL, JL ;

GUERRANT, RL .

INFECTION AND IMMUNITY, 1983, 40 (03) :1198-1203

[9]

IRONS LI, 1988, PATHOGENESIS IMMUNIT, P95

[10]

KEARNEY JF, 1979, J IMMUNOL, V123, P1548

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