SEQUENCE AND CONSERVATION OF GENES AT THE DISTAL END OF THE TRANSFER REGION ON PLASMID-F AND PLASMID-R6-5

被引:16
作者
CRAM, DS [1 ]
LOH, SM [1 ]
CHEAH, KC [1 ]
SKURRAY, RA [1 ]
机构
[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA
基金
澳大利亚研究理事会;
关键词
RECOMBINANT DNA; CONJUGATION; FERTILITY INHIBITION; MOLECULAR EVOLUTION; HOMOLOGY; ESCHERICHIA-COLI; IS3;
D O I
10.1016/0378-1119(91)90469-R
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The nucleotide sequence of the region downstream of transfer gene traI, including, fertility inhibition gene finO, on the conjugative plasmids F and R6-5, has been determined. Analysis of the F sequence revealed two open reading frames (ORF's), ORF248 and ORF186; ORF186 (finO) is interrupted by the insertion of IS3. The R6-5 sequence also contained ORF248 and an intact ORF186, although an additional ORF (ORF286) was located between the two genes. ORF248, which we have designated traX, and ORF186 (finO) are highly conserved on both plasmids. The organisation of these genes indicates that traI and traX on F, and traI, traX and ORF286 on R6-5 are co-transcribed from their respective promoters upstream of traI. Sequences homologous to traX were detected on a range of conjugative F-like plasmids, whereas sequences homologous to ORF286 were only found on plasmids R6-5, R100 and R1. The conservation of traX sequences suggests a functional importance for that gene and/or its product.
引用
收藏
页码:85 / 90
页数:6
相关论文
共 34 条
  • [1] ASSIGNMENT OF TRA CISTRONS TO ECORI FRAGMENTS OF F-SEX FACTOR DNA
    ACHTMAN, M
    SKURRAY, RA
    THOMPSON, R
    HELMUTH, R
    HALL, S
    BEUTIN, L
    CLARK, AJ
    [J]. JOURNAL OF BACTERIOLOGY, 1978, 133 (03) : 1383 - 1392
  • [2] NUCLEOTIDE-SEQUENCE OF THE TRAI (HELICASE-I) GENE FROM THE SEX FACTOR-F
    BRADSHAW, HD
    TRAXLER, BA
    MINKLEY, EG
    NESTER, EW
    GORDON, MP
    [J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (07) : 4127 - 4131
  • [3] CHEAH KC, 1986, J GEN MICROBIOL, V132, P3269
  • [4] FINO SEQUENCES ON CONJUGALLY REPRESSED AND DEREPRESSED F-LIKE PLASMIDS
    CHEAH, KC
    HIRST, R
    SKURRAY, R
    [J]. PLASMID, 1987, 17 (03) : 233 - 239
  • [5] CLONING AND MOLECULAR ANALYSIS OF THE FINO REGION FROM THE ANTIBIOTIC-RESISTANCE PLASMID R6-5
    CHEAH, KC
    RAY, A
    SKURRAY, R
    [J]. PLASMID, 1984, 12 (03) : 222 - 226
  • [6] THE GENBANK-SERVER AT THE UNIVERSITY-OF-HOUSTON
    DAVISON, DB
    CHAPPELEAR, JE
    [J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (06) : 1571 - 1572
  • [7] TRANSCRIPT ANALYSIS OF THE PLASMID R100 TRAJ AND FINP GENES
    DEMPSEY, WB
    [J]. MOLECULAR & GENERAL GENETICS, 1987, 209 (03): : 533 - 544
  • [8] THE FINO GENE OF ANTIBIOTIC-RESISTANCE PLASMID R100
    DEMPSEY, WB
    MCINTIRE, SA
    [J]. MOLECULAR & GENERAL GENETICS, 1983, 190 (03): : 444 - 451
  • [9] IDENTIFYING NONPOLAR TRANSBILAYER HELICES IN AMINO-ACID-SEQUENCES OF MEMBRANE-PROTEINS
    ENGELMAN, DM
    STEITZ, TA
    GOLDMAN, A
    [J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1986, 15 : 321 - 353
  • [10] NATURE OF TRANSFER INHIBITOR OF SEVERAL F-LIKE PLASMIDS
    FINNEGAN, D
    WILLETTS, N
    [J]. MOLECULAR AND GENERAL GENETICS, 1972, 119 (01): : 57 - +