PREPARATION AND BIOLOGICAL PROPERTIES OF NATIVE AND RECOMBINANT CILIARY NEUROTROPHIC FACTOR

被引:24
作者
GUPTA, SK
ALTARES, M
BENOIT, R
RIOPELLE, RJ
DUNN, RJ
RICHARDSON, PM
机构
[1] MCGILL UNIV, DEPT NEUROL & NEUROSURG, MONTREAL H3A 2T5, QUEBEC, CANADA
[2] MCGILL UNIV, DIV ENDOCRINOL, MONTREAL H3A 2T5, QUEBEC, CANADA
[3] MONTREAL GEN HOSP, MONTREAL H3G 1A4, QUEBEC, CANADA
[4] QUEENS UNIV, DIV NEUROL, KINGSTON K7L 3N6, ONTARIO, CANADA
来源
JOURNAL OF NEUROBIOLOGY | 1992年 / 23卷 / 05期
关键词
NEURONAL GROWTH FACTOR; CILIARY NEUROTROPHIC FACTOR; PROTEIN PURIFICATION; NEURONAL SURVIVAL; PARASYMPATHETIC NEURON; RECOMBINANT DNA;
D O I
10.1002/neu.480230504
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
CNTF (ciliary neurotrophic factor), purified from rabbit sciatic nerves by a relatively simple procedure, is bioactive in tissue culture at low picomolar concentration and appears as a doublet on polyacrylamide gel electrophoresis (PAGE). In these nerves, CNTF accounts for more than one-half of the survival-promoting activity on ciliary neurons. The concentration of CNTF in rabbit sciatic nerves is estimated to be 5 nmol'/kg, more than 1000 times higher than would seem to be required to support neurons if the neurotrophic factor were homogeneously distributed. With recombinant DNA technology, rat CNTF has been synthesized in Escherichia coli, purified without denaturating agents, and found to be bioactive at a slightly lower concentration than CNTF extracted from rabbit sciatic nerves. After radioiodination, CNTF retains biological activity but is not specifically internalized and retrogradely transported in motor and sensory axons. In peripheral nerves, ciliary neurotrophic factor differs biologically from nerve growth factor (NGF) by its much higher tissue concentration and apparent lack of internalization by peripheral nerve axons.
引用
收藏
页码:481 / 490
页数:10
相关论文
共 53 条
[1]   DETECTION OF BRAIN-DERIVED NEUROTROPHIC FACTOR-LIKE ACTIVITY IN FIBROBLASTS AND SCHWANN-CELLS - INHIBITION BY ANTIBODIES TO NGF [J].
ACHESON, A ;
BARKER, PA ;
ALDERSON, RF ;
MILLER, FD ;
MURPHY, RA .
NEURON, 1991, 7 (02) :265-275
[2]   INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].
AEBERSOLD, RH ;
LEAVITT, J ;
SAAVEDRA, RA ;
HOOD, LE ;
KENT, SBH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6970-6974
[3]  
ARAKAWA Y, 1990, J NEUROSCI, V10, P3507
[4]   PURIFICATION OF THE CHICK EYE CILIARY NEURONOTROPHIC FACTOR [J].
BARBIN, G ;
MANTHORPE, M ;
VARON, S .
JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) :1468-1478
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]   MODES OF FGF RELEASE INVIVO AND INVITRO [J].
DAMORE, PA .
CANCER AND METASTASIS REVIEWS, 1990, 9 (03) :227-238
[7]   THE RECEPTOR FOR CILIARY NEUROTROPHIC FACTOR [J].
DAVIS, S ;
ALDRICH, TH ;
VALENZUELA, DM ;
WONG, V ;
FURTH, ME ;
SQUINTO, SP ;
YANCOPOULOS, GD .
SCIENCE, 1991, 253 (5015) :59-63
[9]   NEURITE OUTGROWTH ELICITED BY EMBRYONIC CHICK HEART - PARTIAL-PURIFICATION OF THE ACTIVE FACTOR [J].
EBENDAL, T ;
BELEW, M ;
JACOBSON, CO ;
PORATH, J .
NEUROSCIENCE LETTERS, 1979, 14 (01) :91-95
[10]   PURIFICATION AND CHARACTERIZATION OF A TROPHIC FACTOR FOR EMBRYONIC PERIPHERAL NEURONS - COMPARISON WITH FIBROBLAST GROWTH-FACTORS [J].
ECKENSTEIN, FP ;
ESCH, F ;
HOLBERT, T ;
BLACHER, RW ;
NISHI, R .
NEURON, 1990, 4 (04) :623-631