DOUBLE-STAINING ARTIFACT OBSERVED IN CERTAIN INDIVIDUALS DURING DUAL-COLOR IMMUNOPHENOTYPING OF LYMPHOCYTES BY FLOW-CYTOMETRY

被引：10

作者：

EKONG, T ^{[1
]}

GOMPELS, M ^{[1
]}

CLARK, C ^{[1
]}

PARKIN, J ^{[1
]}

PINCHING, A ^{[1
]}

机构：

[1] ST MARYS HOSP,SCH MED,DEPT IMMUNOL,LONDON W2 1PG,ENGLAND

来源：

CYTOMETRY | 1993年 / 14卷 / 06期

关键词：

LYMPHOCYTE SUBSETS; DUAL-COLOR FLOW CYTOMETRY; COEXPRESSION; SERUM FACTOR; GAMMA-GLOBULIN FRACTION;

D O I：

10.1002/cyto.990140613

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An unusual double-staining artefact has been repeatedly observed in certain individuals (both HIV-positive and healthy laboratory controls) during 2-colour immunophenotyping of lymphocytes by flow cytometry after red cell lysis. This artefact can falsely suggest co-expression of CD4 and CD8, as well as some of the other monoclonal antibody pairs commonly used in the typing of lymphocytes. It is caused by a serum factor associated with the ammonium-sulphate-precipitated and gamma-globulin fractions. It appears to be fairly common in the population (being found in 6 out of 105 of our healthy laboratory controls and in 42 out of 247 of our HIV-1 sero-positive subjects); it may be genetically determined, but may be acquired in some. It is a potential source of error in lymphocyte immunophenotyping, which can be avoided by simple adjustments to the recommended techniques. (C) 1993 Wiley-Liss, Inc.

引用

页码：679 / 684

页数：6

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