DETECTION OF CYCLODIENE INSECTICIDE RESISTANCE-ASSOCIATED MUTATIONS BY SINGLE-STRANDED CONFORMATIONAL POLYMORPHISM ANALYSIS

被引:16
作者
COUSTAU, C [1 ]
FFRENCHCONSTANT, R [1 ]
机构
[1] UNIV WISCONSIN,DEPT ENTOMOL,RUSSELL LABS 237,MADISON,WI 53706
来源
PESTICIDE SCIENCE | 1995年 / 43卷 / 04期
关键词
GABA RECEPTOR; INSECTICIDE RESISTANCE; CYCLODIENE INSECTICIDES; RESISTANCE MONITORING; RDL;
D O I
10.1002/ps.2780430404
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Cyclodiene insecticide resistance is associated with replacements of a single amino acid within the putative lining of a delta-aminobutyric acid (GABA)-gated chloride ion channel gene Resistance to dieldrin (Rdl). Only two resistance-associated amino acid replacements have been identified; alanine to serine in Drosophila melanogaster, D. simulans, Aedes aegypti, and Tribolium castaneum and alanine to glycine as a second allele in D. simulans. Here we report that single stranded conformational polymorphism (SSCP) analysis of genomic DNA, amplified by the polymerase chain reaction (PCR) for exon 7 of the Rdl gene, can be used to genotype strains or individuals of all of these insects. This technique also appears simultaneously to distinguish between D. melanogaster and D. simulans, sibling species only reliably identifiable by examination of male genitalia. The relative advantages of this genotyping technique against other PCR-mediated techniques in monitoring for insecticide resistance are discussed.
引用
收藏
页码:267 / 271
页数:5
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