INFLUENCE OF RIBONUCLEOSIDE TRIPHOSPHATES, AND OTHER FACTORS, ON THE FORMATION OF VERY-SALT-STABLE RNA-POLYMERASE SUIII+ TRANSFER RNA (TRANSFER-RNATYR PROMOTER COMPLEXES

被引：6

作者：

DEBENHAM, P ^{[1
]}

机构：

[1] MRC, MOLEC BIOL LAB, CAMBRIDGE, ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 96卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13067.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of a stable RNA‐polymerase · su+III‐tRNA‐promoter complex was found to require sigma factor and the incorporation of ribonucleoside triphosphates which match the 5′ sequence of the su+III tRNA transcript. This complex, stable to at least 2 M KCl, can be retained on a Millipore filter. Its formation closely parallels the extent of transcription obtained from the su+III tRNA promoter in response both to increasing ionic strength and to temperature during incubation of RNA polymerase with the DNA. The RNA‐polymerase · DNA complex retained during this assay therefore appears to relate directly to that formed during promoter‐directed transcription. The formation of RNA‐polymerase · su+III‐tRNA‐promoter complexes is sensitive to the presence of ppGpp. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：535 / 543

页数：9

共 33 条

[1] TYROSINE TRANSFER RNA PRECURSOR MOLECULE POLYNUCLEOTIDE SEQUENCE [J].