DISTRIBUTION OF NEWLY FORMED RIBOSOMAL-PROTEINS IN HELA-CELL FRACTIONS

被引：40

作者：

WARNER, JR ^{[1
]}

机构：

[1] YESHIVA UNIV,ALBERT EINSTEIN COLL MED,DEPT CELL BIOL,BRONX,NY 10461

来源：

JOURNAL OF CELL BIOLOGY | 1979年 / 80卷 / 03期

关键词：

D O I：

10.1083/jcb.80.3.767

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The distribution of newly formed ribosomal proteins between cytoplasmic, nucleoplasmic, and nucleolar fractions of HeLa cells was determined. All but a few of the newly formed ribosomal proteins were concentrated 10- to 50-fold in the nucleolus and two- to fivefold in the nucleoplasm. Nevertheless, substantial amounts were found in the cytoplasm. Pretreatment of cells with actinomycin D to deplete the nucleolar pool of ribosomal precursor RNA had no effect on the concentration of newly formed ribosomal proteins in the nucleus, but did lead to an increased amount in the nucleoplasm at the expense of the nucleolus.

引用

页码：767 / 772

页数：6

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