ACTIVATION OF BENZO(A)PYRENE-7,8-DIHYDRODIOL IN RAT UTERUS - AN INVITRO STUDY

被引：6

作者：

BYCZKOWSKI, JZ ^{[1
]}

KULKARNI, AP ^{[1
]}

机构：

[1] UNIV S FLORIDA,COLL PUBL HLTH,TOXIKOL PROGRAM,13301 BRUCE B DOWNS BLVD,MHH-104,TAMPA,FL 33612

来源：

JOURNAL OF BIOCHEMICAL TOXICOLOGY | 1990年 / 5卷 / 03期

关键词：

Benzo(apyrene‐7,8‐dihydrodiol; Co‐oxidation; Hydrogen Peroxide; Peroxidase; Rat; Uterus;

D O I：

10.1002/jbt.2570050302

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Peroxidatic metabolism of benzo(a) pyrene‐7,8‐dihydrodiol by calcium containing extracts of rat uteri was investigated. Covalently bound and soluble metabolites of benzo(a)pyrene‐7,8‐dihydrodiol were quantitated by radiometry and high performance liquid chromatography, respectively Uterine extracts incubated with benzo(a)pyrene‐7,8‐dihydrodiol activated this proximate mutagen to protein binding metabolite(s). Hydrogen peroxide increased the protein binding and yielded a substantial amount of benzo(a)pyrene‐trans‐anti‐tetrahydrotetrol, suggesting the peroxyl‐type free‐radical epoxidation process. The results indicate that rat uterine peroxidase is able to catalyze free‐radical activation of benzo (a)pyrene‐7,8‐dihydrodiol by epoxidation to its 9,10‐dihydrodiolepoxide, a known ultimate mutagen and carcinogen. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：139 / 145

页数：7

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