CONTRIBUTION OF THE CELL-SURFACE-ASSOCIATED ENZYME IN THE ZIMMERMANN-ROSSELET ASSAY OF OUTER-MEMBRANE PERMEABILITY TO BETA-LACTAM ANTIBIOTICS

被引：14

作者：

LIU, W ^{[1
]}

NIKAIDO, H ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,229 STANLEY HALL,BERKELEY,CA 94720

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1991年 / 35卷 / 01期

关键词：

D O I：

10.1128/AAC.35.1.177

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Analysis of beta-lactam hydrolysis by intact cells of several strains of enteric bacteria suggests that a significant fraction of beta-lactamase molecules is present at a location directly accessible for the substrates in the medium. We propose a method to correct for the error introduced by these "surface-exposed" enzymes in the Zimmermann-Rosselet assay of outer membrane permeability.

引用

页码：177 / 179

页数：3

共 8 条

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