PARTICLE CONCENTRATION FLUORESCENCE IMMUNOASSAY OF GENTAMICIN

被引：4

作者：

VARMANELSON, P

NELSON, JE

BELKNAP, SM

机构：

[1] LOYOLA UNIV,MED CTR,BLDG 54,ROOM 109,2160 S 1ST AVE,MAYWOOD,IL 60153

[2] ST XAVIERS COLL,CHICAGO,IL

[3] EDWARD HINES JR VET ADM HOSP,HINES,IL

来源：

THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 03期

关键词：

FLUORESCENCE POLARIZATION IMMUNOASSAY; AMINOGLYCOSIDES; PARTICLE CONCENTRATION FLUORESCENCE IMMUNOASSAY;

D O I：

10.1097/00007691-199105000-00013

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We report the development of a particle concentration fluorescence immunoassay (PCFIA) for gentamicin using commercially available reagents. The solid phase consists of 0.8-mu-m polystyrene spheres to which gentamicin antibody is noncovalently bound. Fluorescein-labeled gentamicin serves as the tracer. The assay is carried out on 96-well reaction plates and read using an automated instrument. The assay is accurate over the range of gentamicin concentrations commonly encountered in clinical practice, with day-to-day coefficients of variation of 3-7%. As little as 0.01 mg/L of gentamicin can be detected when sample predilution is omitted. PCFIA is compared with Abbott TDX fluorescence polarization immunoassay for the measurement of serum gentamicin concentrations in routine clinical specimens.

引用

页码：260 / 262

页数：3

共 8 条

[1] QUANTIFICATION OF ANTIGEN-ANTIBODY REACTION BY POLARIZATION OF FLUORESCENCE [J].