THE RNE GENE IS THE STRUCTURAL GENE FOR THE PROCESSING ENDORIBONUCLEASE RNASE-E OF ESCHERICHIA-COLI

被引：6

作者：

CHAUHAN, AK ^{[1
]}

APIRION, D ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,BOX 8230,ST LOUIS,MO 63110

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 228卷 / 1-2期

关键词：

RNASE-E SUBSTRATES; T7 RNA POLYMERASE; 5S RIBOSOMAL-RNA; RNA-I; RNASE-E STRUCTURAL GENE;

D O I：

10.1007/BF00282446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using T7 RNA polymerase and specific constructs derived from 5S rRNA and RNA I genes, we generated substrates for the RNA processing enzyme RNase E. Using these substrates we have shown that a 3.2 kb DNA fragment that complements the rne-3071 mutation can express RNase E activity. We also found that T7 RNA polymerase terminates within the 5S rRNA gene.

引用

页码：49 / 54

页数：6

共 24 条

[1] SEQUENCING AND EXPRESSION OF THE RNE GENE OF ESCHERICHIA-COLI [J].

CHAUHAN, AK ;

MICZAK, A ;

TARASEVICIENE, L ;

APIRION, D .

NUCLEIC ACIDS RESEARCH, 1991, 19 (01) :125-129

[2]

DALLMANN G, 1987, MOL GEN LIFE SCI ADV, V6, P99

[3]

Davis L, 1986, BASIC METHODS MOL BI

[4]

Elford R M, 1983, J Mol Biol, V168, P557, DOI 10.1016/S0022-2836(83)80301-5

[5]

GEGENHEIMER P, 1977, J BIOL CHEM, V252, P3064

[6] STRUCTURAL-ANALYSIS AND INVITRO PROCESSING TO P5 RIBOSOMAL-RNA OF A 9S RNA MOLECULE ISOLATED FROM AN RNE MUTANT OF ESCHERICHIA-COLI [J].

GHORA, BK ;

APIRION, D .

CELL, 1978, 15 (03) :1055-1066

[7] THE SYNTHESIS OF SOME PROTEINS IS AFFECTED IN RNA PROCESSING MUTANTS OF ESCHERICHIA-COLI [J].

GITELMAN, DR ;

APIRION, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 96 (03) :1063-1070

[8] INACTIVATION OF THE RIBONUCLEIC ACID-PROCESSING ENZYME RIBONUCLEASE-E BLOCKS CELL-DIVISION [J].

GOLDBLUM, K ;

APRIRION, D .

JOURNAL OF BACTERIOLOGY, 1981, 146 (01) :128-132

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 3 →