COMPARISON OF FLOW-CYTOMETRY AND EPIFLUORESCENCE MICROSCOPY FOR COUNTING BACTERIA IN AQUATIC ECOSYSTEMS

被引：41

作者：

MONFORT, P

BALEUX, B

机构：

[1] COMMISS EUROPEAN COMMUNITIES,JOINT RES CTR,INST ENVIRONM,I-21020 ISPRA,ITALY

[2] UNIV MONTPELLIER 2,HYDROBIOL MARINE & CONTINENTALE LAB,CNRS,URA 1355,F-34095 MONTPELLIER 05,FRANCE

来源：

CYTOMETRY | 1992年 / 13卷 / 02期

关键词：

BACTERIA; ENUMERATION; FLOW CYTOMETRY; EPIFLUORESCENCE MICROSCOPY; AQUATIC ECOSYSTEMS;

D O I：

10.1002/cyto.990130213

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cytometry was used to count bacterial cells from diverse origins: one strain of E. coli, one sample of lake water, and 18 samples of estuary water. To verify the accuracy and the precision of this technique, total bacteria counts made by flow cytometry were compared with counts by direct observation using epifluorescence microscopy. The results of this study showed that flow cytometry was a reliable technique for counting a mixture of bacteria in samples from aquatic ecosystems.

引用

页码：188 / 192

页数：5

共 13 条

[1] METHOD FOR FLOW CYTOMETRIC DETECTION OF LISTERIA-MONOCYTOGENES IN MILK [J].