POLIOVIRUS ANTIGENIC HYBRIDS SIMULTANEOUSLY EXPRESSING ANTIGENIC DETERMINANTS FROM ALL 3 SEROTYPES

被引：21

作者：

MURDIN, AD ^{[1
]}

LU, HH ^{[1
]}

MURRAY, MG ^{[1
]}

WIMMER, E ^{[1
]}

机构：

[1] SUNY STONY BROOK, DEPT MICROBIOL, STONY BROOK, NY 11794 USA

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-3-607

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have constructed six hybrid polioviruses (PVs) modified to express PV type 2 and type 3 antigenic determinants on a PV type 1 (Mahoney) capsid. The hybrids were modified in neutralizing antigenic site (NAg) I and/or NAgII. They were viable, but impaired for growth in comparison to PV1 (Mahoney). Some hybrids modified to express type 2 and type 3 NAgI determinants simultaneously displayed some type 2 but no type 3 antigenicity (in addition to type 1 antigenicity associated with other antigenic sites). Hybrids modified to express a type 2 NAgI determinant and a type 3 NAgII determinant, or vice versa, displayed antigenic characteristics of all three serotypes, although expression of the modified NAgII determinant was weak. We conclude that it is possible to construct a viable hybrid PV simultaneously modified in NAgI and NAgII which expresses antigenic determinants of all three serotypes.

引用

页码：607 / 611

页数：5

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