DETERMINATION OF RISPERIDONE AND 9-HYDROXYRISPERIDONE IN PLASMA, URINE AND ANIMAL-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：53

作者：

WOESTENBORGHS, R

LORREYNE, W

VANROMPAEY, F

HEYKANTS, J

机构：

[1] Department of Drug Metabolism and Pharmacokinetics, Janssen Research Foundation, B-2340 Beerse

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80556-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method has been developed for the simultaneous determination of the new anti-psychotic risperidone and its major metabolite 9-hydroxyrisperidone in plasma, urine and animal tissues. The alkalinized plasma samples were extracted with ethyl acetate and further purified prior to reversed-phase chromatography with ultraviolet detection at 280 rum. The method could also be applied to urine samples and animal tissue homogenates. Quantification limits were 2 ng/ml for plasma and urine and 10 ng/g for animal tissue. The method was applied to pharmacokinetic studies in experimental animals, human volunteers and patients.

引用

页码：223 / 230

页数：8