APPLICABILITY OF REPORTER GENES TO CARNATION TRANSFORMATION

被引：10

作者：

VAINSTEIN, A ^{[1
]}

FISHER, M ^{[1
]}

ZIV, M ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM, FAC AGR, OTTO WARBURG CTR BIOTECHNOL AGR, DEPT AGR BOT, IL-76100 REHOVOT, ISRAEL

来源：

HORTSCIENCE | 1993年 / 28卷 / 11期

关键词：

DIANTHUS-CARYOPHYLLUS; 4-METHYLUMBELLIFERONE; 4-METHYLUMBELLIFERYL GLUCURONIDE; PHENOLIC COMPOUNDS; POLYVINYLPOLYPYRROLIDONE; CHLORAMPHENICOL ACETYLTRANSFERASE; BETA-GLUCURONIDASE;

D O I：

10.21273/HORTSCI.28.11.1122

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The applicability of beta-glucuronidase and chloramphenicol acetyltransferase reporter genes to a carnation (Dianthus caryophyllus L.) transformation procedure was analyzed. Transgenic tobacco (Nicotiana tabacum L.) plants expressing the respective reporter genes were prepared and used as the enzyme source. Carnation leaf extract strongly inhibited enzymatic activity of beta-glucuronidase, but not that of chloramphenicol acetyltransferase. One or more carnation phenolic compounds, acting in a noncompetitive manner, is suggested as the cause of the observed inhibition of fluorometrically assayed beta-glucuronidase activity. This inhibition was eliminated by treating the carnation leaf extract with polyvinylpolypyrrolidone.

引用

页码：1122 / 1124

页数：3

共 19 条

[1] DEVELOPMENT OF PLANT PROMOTER EXPRESSION VECTORS AND THEIR USE FOR ANALYSIS OF DIFFERENTIAL ACTIVITY OF NOPALINE SYNTHASE PROMOTER IN TRANSFORMED TOBACCO CELLS [J].