FINE-STRUCTURAL INVESTIGATION OF RAT-BRAIN MICROVASCULAR ENDOTHELIAL-CELLS - TIGHT JUNCTIONS AND VESICULAR STRUCTURES IN FRESHLY ISOLATED AND CULTURED PREPARATIONS

A comparison was made between endothelial cells in freshly-isolated rat brain microvessels, and following culture of the cells for 1-10 days during growth to confluence. Attention focused on tight junctions and vesicular structures, as seen in thin sections and freeze-fracture replicas. Freshly-isolated vessels had an abnormal appearance, with a profusion of luminal microvillar professes, and extensive cytoplasmic vacuolation. There were numerous vesicular profiles, reaching a density of similar to 60 mu m(-2), and with a large proportion open to the surface, as shown by labelling with cationized ferritin at 4 degrees C for 5 min. Junctional zones were relatively loosely organized, with evidence for some cell:cell separation, as well as some residual tight junctional sites within zonula adhaerens junctions. In freeze-fracture replicas, junctional strands showed segments of tightly packed intramembrane particles, generally on the P face. After 1 day in culture, the cells appeared more normal, with no vacuolation or luminal processes. Vesicles were still numerous, some associated with junctional zones, while tight junctions were relatively sparse; freeze-fracture showed some incomplete tight junctional strands, with some of the intramembrane particles fracturing onto the E face. The double offset fibrillar nature of the strands could occasionally be seen. Cells cultured for 4 and 10 days showed a progressive increase in the completeness of the junctional zone, with more tight junctional contacts within the length of the adhaerens junction, and an aggregation of microfilaments in the underlying cytoplasm. The number of vesicular profiles declined, and they were progressively excluded from the junctional zone. These observations have relevance for studies on the physiology of the brain endothelium in vitro, and for comparisons with the in vivo condition.