FLUORESCENCE POLARIZATION IMMUNOASSAY EMPLOYING IMMOBILIZED ANTIBODY

被引：15

作者：

TSURUOKA, M ^{[1
]}

TAMIYA, E ^{[1
]}

KARUBE, I ^{[1
]}

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,MEGURO KU,TOKYO 153,JAPAN

来源：

BIOSENSORS & BIOELECTRONICS | 1991年 / 6卷 / 06期

关键词：

BOUND-FREE SEPARATION; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENCE POLARIZATION; IMMOBILIZED ANTIBODY; IMMUNOASSAY; IMMUNOGLOBULIN-G; LATEX; MOLECULAR WEIGHT; SILVER COLLOID;

D O I：

10.1016/0956-5663(91)85047-Z

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The use of an antibody immobilized on latex or silver colloid in fluorescence polarization immunoassay (FPI) is assessed. In FPI it is possible to detect antigens of high molecular weight because the molecular weight of the antibody is effectively increased. In the assay for rabbit immunoglobulin G a limit of detection lower by two orders of magnitude and an assay range wider by one order of magnitude can be obtained in comparison with conventional FPI. The detection limit is 10(-10) mol 1(-1) and the total assay time for one sample is 8 min. This assay combines a low detection limit with a short assay time.

引用

页码：501 / 505

页数：5

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