THE INVITRO METABOLIC-ACTIVATION OF DIBENZ[A,H]ANTHRACENE, CATALYZED BY RAT-LIVER MICROSOMES AND EXAMINED BY P-32 POSTLABELING

被引：14

作者：

LECOQ, S ^{[1
]}

SHE, MN ^{[1
]}

GROVER, PL ^{[1
]}

PLATT, KL ^{[1
]}

OESCH, F ^{[1
]}

PHILLIPS, DH ^{[1
]}

机构：

[1] UNIV MAINZ,INST TOXICOL,W-6500 MAINZ,GERMANY

来源：

CANCER LETTERS | 1991年 / 57卷 / 03期

关键词：

DIBENZ[A; H]ANTHRACENE; DNA ADDUCTS; P-32-POSTLABELING;

D O I：

10.1016/0304-3835(91)90166-F

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA has been incubated in vitro with dibenz[a,h]anthracene (DB[a,h]A) and the related 5,6-diol and 3,4-diol in the presence of 3-methylcholanthrene- or Aroclor 1254-induced rat liver microsomes. After incubation, the DNA was extracted and examined for the presence of aromatic adducts using the nuclease P1 modification of the P-32-postlabelling technique. The maps of PEI-cellulose plates and autoradiography showed that 92 % of the radioactivity contained in DB[a,h]A-DNA adduct spots is derived from the related 3,4-diol and that about 50 % of the adducts may be formed following the conversion of this diol to the bay-region anti- and syn-3,4-diol 1,2-oxides.

引用

页码：261 / 269

页数：9

共 23 条

[1]

BUENING MK, 1979, CANCER RES, V39, P1310

[2]

CONNEY AH, 1982, CANCER RES, V42, P4875

[3] NONRANDOM BINDING OF THE CARCINOGEN N-HYDROXY-2-ACETYLAMINOFLUORENE TO REPETITIVE SEQUENCES OF RAT-LIVER DNA INVIVO [J].