CONTINUOUS HYDROLYSIS OF BETA-CASEIN IN A MEMBRANE REACTOR - PREPARATION OF A BIOACTIVE PEPTIDE

Continuous Stirred Tank Membrane Reactor was used to investigate the continuous and selective extraction of a bioactive peptide beta-CN (193-209) from bovine beta-casein/chymosin hydrolysate. It was shown that the feasibility of the process depends on the nature and the area of ultrafiltration membrane used. With an inorganic (carbon-zirconia) membrane, high retention of the peptide constitutes a limit to the operation. However, when the reactor was equipped with a cellulosic type membrane, satisfactory transmission of the peptide was obtained. As shown by RP-HPLC and mass spectrometry analysis, only beta-CN (193-209) permeates through the membrane.