IMMUNE-RESPONSE OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS, WALBAUM) TO AEROMONAS-HYDROPHILA

被引:32
作者
LOGHOTHETIS, PN
AUSTIN, B
机构
[1] Division of Aquaculture, Department of Biological Sciences, Heriot-Watt University, Edinburgh, EH14 4AS Scotland, Riccarton
关键词
ONCORHYNCHUS-MYKISS; RAINBOW TROUT; AEROMONAS HYDROPHILA; IMMUNE RESPONSE; MACROGLOBULINS;
D O I
10.1006/fsim.1994.1022
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Rainbow trout (Oncorhynchus mykiss Walbaum), immunised by injection, immersion and orally with killed and live cells of Aeromonas hydrophila, produced antibodies in the serum, bile, skin and gut mucus, and skin and muscle extracts. The antibodies were of a low affinity macroglobulin class, sensitive to 2-mercaptoethanol, and of a structure comprising heavy and light chains. These antibodies reacted maximally with formalised cells and lipopolysaccharide (LPS), and to a lesser extent with exopolysaccharide (EPS), flagella, S-layer protein and a haemolytic component of the extracellular products (ECP) of A. hydrophila. © 1994 Academic Press. All rights reserved.
引用
收藏
页码:239 / 254
页数:16
相关论文
共 44 条
[1]  
Al-Harbi A.H., Austin B., The immune response of turbot, Scophthalmus maximus (L.), to lipopolysaccharide from a fish-pathogenic Cytophaga-like bacterium, Journal of Fish Diseases, 15, pp. 449-452, (1992)
[2]  
Allen D.A., Austin B., Colwell R.R., Numerical taxonomy of bacterial isolates associated with a freshwater fishery, Journal of General Microbiology, 129, pp. 2043-2062, (1983)
[3]  
Anders E., Experimental oral immunization of rainbow trout and eel against Vibrio anguillarum, Fischerei-Forschung, Rostock, 16, pp. 53-54, (1978)
[4]  
Austin B., Austin D.A., Bacterial Fish Pathogens, Disease in Farmed and Wild Fish, (1987)
[5]  
Austin D.A., McIntosh D., Austin B., Taxonomy of fish associated Aeromonas spp, With the description of Aeromonas salmonicida subsp. Smithia subsp. Nov, Systematic and Applied Microbiology, 11, pp. 277-290, (1989)
[6]  
Baba T., Imamura J., Izawa K., Ikeda K., Immune potection in the carp, Cyprinus carpio L, after immunization with Aeromonas hy drop hila crude lipopolysaccharide, Journal of Fish Diseases, 11, pp. 237-244, (1988)
[7]  
Baba T., Imamura J., Izawa K., Ikeda K., Cell-mediated protection in carp, Cyprinus carpio L, against Aeromonas hydrophila crude lipopolysaccharide, Journal of Fish Diseases, 11, pp. 171-178, (1986)
[8]  
Dooley J.S.G., McCubbin W.D., Kay C.M., Trust T.J., Isolation and biochemical characterization of the S-layer protein from a pathogenic Aeromonas hydrophila strain, Journal of Bacteriology, 170, pp. 2631-2638, (1988)
[9]  
Filip C., Fletcher G., Wulff J.L., Earhart C.F., Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate, Journal of Bacteriology, 115, pp. 717-722, (1973)
[10]  
Fletcher T.C., Grant P.T., Immunoglobulins in the serum and mucus of plaice (Pleuronectes platessa), Biochemical Journal, 115, (1969)