CROSS-NEUTRALIZING ACTIVITY AGAINST DIVERGENT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES INDUCED BY THE GP41 SEQUENCE ELDKWAS

被引：266

作者：

MUSTER, T ^{[1
]}

GUINEA, R ^{[1
]}

TRKOLA, A ^{[1
]}

PURTSCHER, M ^{[1
]}

KLIMA, A ^{[1
]}

STEINDL, F ^{[1
]}

PALESE, P ^{[1
]}

KATINGER, H ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT MICROBIOL,NEW YORK,NY 10029

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 06期

关键词：

D O I：

10.1128/JVI.68.6.4031-4034.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previously we identified the highly conserved amino acids Glu-Leu-Asp-Lys-Trp-Ala (ELDKWA) on the ectodomain of gp41 as the epitope of a neutralizing monoclonal antibody (2F5) directed against human immunodeficiency virus type 1. In the present study, the sequence defining the epitope was introduced into the loop of antigenic site B of the influenza virus hemagglutinin. The resulting chimeric virus was able to elicit ELDKWA-specific immunoglobulins G and A in antisera of mice. Moreover, the distantly related human immunodeficiency virus type 1 isolates MN, RF, and IIIB were neutralized by these antisera. These data suggest that this conserved B-cell epitope is a promising candidate for inclusion in a vaccine against AIDS. The results also show that influenza virus can be used to effectively present the antigenic structure of this B-cell epitope.

引用

页码：4031 / 4034

页数：4

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