FABMS DERIVATIZATION STRATEGIES FOR THE ANALYSIS OF HEPARIN-DERIVED OLIGOSACCHARIDES

被引：32

作者：

KHOO, KH

MORRIS, HR

MCDOWELL, RA

DELL, A

MACCARANA, M

LINDAHL, U

机构：

[1] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AZ,ENGLAND

[2] SWEDISH UNIV AGR SCI,CTR BIOMED,S-75123 UPPSALA,SWEDEN

来源：

CARBOHYDRATE RESEARCH | 1993年 / 244卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0008-6215(83)85002-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Derivatisation/FABMS strategies applicable to the structure analysis of low microgramme quantities of heparin-derived oligosaccharides are described. Negative and positive FAB data from permethyl derivatives and positive FAB data from the products of subsequent methanolysis are reported for sulfated tetrasaccharides prepared by nitrous acid degradation of heparin. The preparation and FAB behaviour of acetylated derivatives of sulfated oligosaccharides are described for the first time, and the stability of the sulfate groups to base-catalysed acetylation is demonstrated. The acetylation/FABMS methodology, which yields high quality data, shows promise for the characterisation of a wide range of sulfated glycoconjugates.

引用

页码：205 / 223

页数：19

共 26 条

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