THE GENE ENCODING THE PERIPLASMIC CYCLOPHILIN HOMOLOG, PPLASE-A, IN ESCHERICHIA-COLI, IS EXPRESSED FROM 4 PROMOTERS, 3 OF WHICH ARE ACTIVATED BY THE CAMP-CRP COMPLEX AND NEGATIVELY REGULATED BY THE CYTR REPRESSOR

被引:18
作者
NORREGAARDMADSEN, M
MYGIND, B
PEDERSEN, R
VALENTINHANSEN, P
SOGAARDANDERSEN, L
机构
[1] ODENSE UNIV, DEPT MOLEC BIOL, DK-5230 ODENSE M, DENMARK
[2] UNIV COPENHAGEN, DEPT BIOL CHEM, DK-1307 COPENHAGEN K, DENMARK
关键词
D O I
10.1111/j.1365-2958.1994.tb01333.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rot gene in Escherichia coli encodes PPlase A, a periplasmic peptidyl-prolyl cis-trans isomerase with homology to the cyclophilin family of proteins. Here it is demonstrated that rot is expressed in a complex manner from four overlapping promoters and that the rot regulatory region is unusually compact, containing a close array of sites for DNA-binding proteins. The three most upstream rot promoters are activated by the global gene regulatory cAMP-CRP complex and negatively regulated by the CytR repressor protein. Activation of these three promoters occurs by binding of cAMP-CRP to two sites separated by 53 bp. Moreover, one of the cAMP-CRP complexes is involved in the activation of both a Class I and a Class II promoter. Repression takes place by the formation of a CytR/cAMP-CRP/DNA nucleoprotein complex consisting of the two cAMP-CRP molecules and CytR bound in between. The two regulators bind co-operatively to the DNA overlapping the three upstream promoters, simultaneously quenching the cAMP-CRP activator function. These results expand the CytR regulon to include a gene whose product has no known function in ribo- and deoxyribonucleoside catabolism or transport.
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收藏
页码:989 / 997
页数:9
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